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Synthesis of Silver Nanoparticles Using Dichloromethane-Methanol Flower Extract of Chrysanthemum cinerariaefolium and Its Antibacterial Activity

Received: 16 June 2020     Accepted: 24 August 2020     Published: 23 February 2021
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Abstract

Nanotechnology is an emerging field that has opened new horizons in nanomedicine. The use of silver nanoparticles is attracting much interest because of their antibacterial activity. This study involved synthesis of silver nanoparticles using Chrysanthemum cinerariaefolium flowers dichloromethane-methanol crude extract. The synthesized silver nanoparticles (Ag NPs) were characterized using UV-Vis spectroscopy, SEM, EDX, TEM and FTIR. The antibacterial potential of the nanoparticles was ascertained against methicillin-resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa, Staphylococcus aureus and Shigella sonnei. This was followed by phytochemical analyses of the crude extracts. The Ag NPs were generally spherical as observed in the SEM and TEM micrographs with an average size of 26.98 nm. The UV- absorption spectrum revealed prominent peak at 430 nm while EDX analysis showed the percentage abundance of silver nanoparticle at (81.33%). The FTIR spectroscopy confirmed absorption bands of various functional groups on the surface of Ag NPs. The absorption bands were at 3472.88 cm-1, 3190.67 cm-1, 1646.61 cm-1, 1405.08 cm-1, 1109.32 cm-1 and 518.64 cm-1. Antibacterial potential of the synthesized Ag NPs showed that they were more active on S. aureus with an MIC of 31.25 µg/ml. The phytochemicals observed in the crude extracts that could have been responsible for reducing silver ions into silver nanoparticles were flavonoids, phenols, tannins and glycosides.

Published in American Journal of Nano Research and Applications (Volume 9, Issue 1)
DOI 10.11648/j.nano.20210901.11
Page(s) 1-8
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2021. Published by Science Publishing Group

Keywords

Nanoparticles, Nanotechnology Antibacterial, Phytochemicals

References
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    Caroline Jepchirchir Kosgei, Festus Tolo, Josphat Clement Matasyoh, Meshack Obonyo, Peter Mwitari, et al. (2021). Synthesis of Silver Nanoparticles Using Dichloromethane-Methanol Flower Extract of Chrysanthemum cinerariaefolium and Its Antibacterial Activity. American Journal of Nano Research and Applications, 9(1), 1-8. https://doi.org/10.11648/j.nano.20210901.11

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    ACS Style

    Caroline Jepchirchir Kosgei; Festus Tolo; Josphat Clement Matasyoh; Meshack Obonyo; Peter Mwitari, et al. Synthesis of Silver Nanoparticles Using Dichloromethane-Methanol Flower Extract of Chrysanthemum cinerariaefolium and Its Antibacterial Activity. Am. J. Nano Res. Appl. 2021, 9(1), 1-8. doi: 10.11648/j.nano.20210901.11

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    AMA Style

    Caroline Jepchirchir Kosgei, Festus Tolo, Josphat Clement Matasyoh, Meshack Obonyo, Peter Mwitari, et al. Synthesis of Silver Nanoparticles Using Dichloromethane-Methanol Flower Extract of Chrysanthemum cinerariaefolium and Its Antibacterial Activity. Am J Nano Res Appl. 2021;9(1):1-8. doi: 10.11648/j.nano.20210901.11

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  • @article{10.11648/j.nano.20210901.11,
      author = {Caroline Jepchirchir Kosgei and Festus Tolo and Josphat Clement Matasyoh and Meshack Obonyo and Peter Mwitari and Lucia Keter and James Jorum Owuor and Moses Ollengo and Beatrice Irungu},
      title = {Synthesis of Silver Nanoparticles Using Dichloromethane-Methanol Flower Extract of Chrysanthemum cinerariaefolium and Its Antibacterial Activity},
      journal = {American Journal of Nano Research and Applications},
      volume = {9},
      number = {1},
      pages = {1-8},
      doi = {10.11648/j.nano.20210901.11},
      url = {https://doi.org/10.11648/j.nano.20210901.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.nano.20210901.11},
      abstract = {Nanotechnology is an emerging field that has opened new horizons in nanomedicine. The use of silver nanoparticles is attracting much interest because of their antibacterial activity. This study involved synthesis of silver nanoparticles using Chrysanthemum cinerariaefolium flowers dichloromethane-methanol crude extract. The synthesized silver nanoparticles (Ag NPs) were characterized using UV-Vis spectroscopy, SEM, EDX, TEM and FTIR. The antibacterial potential of the nanoparticles was ascertained against methicillin-resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa, Staphylococcus aureus and Shigella sonnei. This was followed by phytochemical analyses of the crude extracts. The Ag NPs were generally spherical as observed in the SEM and TEM micrographs with an average size of 26.98 nm. The UV- absorption spectrum revealed prominent peak at 430 nm while EDX analysis showed the percentage abundance of silver nanoparticle at (81.33%). The FTIR spectroscopy confirmed absorption bands of various functional groups on the surface of Ag NPs. The absorption bands were at 3472.88 cm-1, 3190.67 cm-1, 1646.61 cm-1, 1405.08 cm-1, 1109.32 cm-1 and 518.64 cm-1. Antibacterial potential of the synthesized Ag NPs showed that they were more active on S. aureus with an MIC of 31.25 µg/ml. The phytochemicals observed in the crude extracts that could have been responsible for reducing silver ions into silver nanoparticles were flavonoids, phenols, tannins and glycosides.},
     year = {2021}
    }
    

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  • TY  - JOUR
    T1  - Synthesis of Silver Nanoparticles Using Dichloromethane-Methanol Flower Extract of Chrysanthemum cinerariaefolium and Its Antibacterial Activity
    AU  - Caroline Jepchirchir Kosgei
    AU  - Festus Tolo
    AU  - Josphat Clement Matasyoh
    AU  - Meshack Obonyo
    AU  - Peter Mwitari
    AU  - Lucia Keter
    AU  - James Jorum Owuor
    AU  - Moses Ollengo
    AU  - Beatrice Irungu
    Y1  - 2021/02/23
    PY  - 2021
    N1  - https://doi.org/10.11648/j.nano.20210901.11
    DO  - 10.11648/j.nano.20210901.11
    T2  - American Journal of Nano Research and Applications
    JF  - American Journal of Nano Research and Applications
    JO  - American Journal of Nano Research and Applications
    SP  - 1
    EP  - 8
    PB  - Science Publishing Group
    SN  - 2575-3738
    UR  - https://doi.org/10.11648/j.nano.20210901.11
    AB  - Nanotechnology is an emerging field that has opened new horizons in nanomedicine. The use of silver nanoparticles is attracting much interest because of their antibacterial activity. This study involved synthesis of silver nanoparticles using Chrysanthemum cinerariaefolium flowers dichloromethane-methanol crude extract. The synthesized silver nanoparticles (Ag NPs) were characterized using UV-Vis spectroscopy, SEM, EDX, TEM and FTIR. The antibacterial potential of the nanoparticles was ascertained against methicillin-resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa, Staphylococcus aureus and Shigella sonnei. This was followed by phytochemical analyses of the crude extracts. The Ag NPs were generally spherical as observed in the SEM and TEM micrographs with an average size of 26.98 nm. The UV- absorption spectrum revealed prominent peak at 430 nm while EDX analysis showed the percentage abundance of silver nanoparticle at (81.33%). The FTIR spectroscopy confirmed absorption bands of various functional groups on the surface of Ag NPs. The absorption bands were at 3472.88 cm-1, 3190.67 cm-1, 1646.61 cm-1, 1405.08 cm-1, 1109.32 cm-1 and 518.64 cm-1. Antibacterial potential of the synthesized Ag NPs showed that they were more active on S. aureus with an MIC of 31.25 µg/ml. The phytochemicals observed in the crude extracts that could have been responsible for reducing silver ions into silver nanoparticles were flavonoids, phenols, tannins and glycosides.
    VL  - 9
    IS  - 1
    ER  - 

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Author Information
  • Department of Biochemistry, Faculty of Science, Egerton University, Nakuru, Kenya

  • Centre for Traditional Medicine and Drugs Research (CTMDR), Kenya Medical Research Institute, Nairobi, Kenya

  • Department of Chemistry, Faculty of Science, Egerton University, Egerton, Kenya

  • Department of Biochemistry, Faculty of Science, Egerton University, Nakuru, Kenya

  • Centre for Traditional Medicine and Drugs Research (CTMDR), Kenya Medical Research Institute, Nairobi, Kenya

  • Centre for Traditional Medicine and Drugs Research (CTMDR), Kenya Medical Research Institute, Nairobi, Kenya

  • Department of Chemical Science and Technology, School of Chemistry and Material Science, Faculty of Applied and Sciences and Technology, Technical University of Kenya, Nairobi, Kenya

  • Department of Chemistry, School of Science, Dedan Kimathi University of Technology, Nyeri, Kenya

  • Centre for Traditional Medicine and Drugs Research (CTMDR), Kenya Medical Research Institute, Nairobi, Kenya

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